Second order parameter-uniform convergence for a finite difference method for a singularly perturbed linear reaction-diffusion system

A singularly perturbed linear system of second order ordinary differential equations of reaction-diffusion type with given boundary conditions is considered. The leading term of each equation is multiplied by a small positive parameter. These singular perturbation parameters are assumed to be distinct. The components of the solution exhibit overlapping layers. Shishkin piecewise-uniform meshes are introduced, which are used in conjunction with a classical finite difference discretisation, to construct a numerical method for solving this problem. It is proved that the numerical approximations obtained with this method is essentially second order convergent uniformly with respect to all of the parameters

STERILITY TESTING PROCEDURE OF OPHTHALMIC OCUSERT ACICLOVIR USED FOR TREATING HERPES SIMPLEX VIRUS

  Objective: The present work focuses on sterility studies of prepared aciclovir ocusert which is so essential for ophthalmic preparations. According to Indian Pharmacopoeia, the sterility test was performed. Ocuserts are sterile preparations which are placed into cul-de-sac or conjunctival sac. Ophthalmic inserts offer many advantages over conventional dosage forms such as increased ocular residence time, possibility of releasing drugs at a slow and constant rate and accurate dosing. Ocuserts are formulated for treating external ocular diseases such as conjuctivitis, corneal ulcer, and keratoconjuctivitis. Ophthalmic preparations contaminated with microorganisms cause corneal damage and finally blindness, especially if the microorganism is Pseudomonas aeruginosa. Ophthalmic preparations should be manufactured in aseptic condition and to be sterilized before packing.Methods: According to Indian Pharmacopoeia, the official sterility test for ocusert was performed for detecting the presence of microbes. The selected F2 formulation which shows controlled drug release after 24 hrs was selected for the sterility test. The F2 formulation was subjected to ultraviolet radiation for sterilization. The sterilized ocusert and unsterilized ocusert were placed in fluid thioglycollate medium and incubated for 7 days at 20-25oC.Results: After 7 days of incubation, the sterilized ocusert shows no microbial growth and unsterilized ocusert shows microbial growth. The prepared aciclovir ocuserts were found to be sterile after the completion of official sterility test.Conclusion: The sterility studies conclude after 7 days of incubation period; there was no appearance of turbidity which indicates the prepared formulation F2 was found to be sterile

EFFECT OF YOGA AND BRISK WALKING ON SELECTED PHYSIOLOGICAL VATIABLES (BLOOD PRESSURE) OF NON-INSULIN DEPENDENT DIBETIC PATIENTS

The purpose of the study was to find out the effect of yoga and brisk walking on selected physiological variables of Non Insulin Dependent Diabetic Patients. For this purpose, Forty five (N=45) aged between 35 to 45 years working men in a private company at Hosur, Tamilnadu, India were randomly selected as subjects. The training programme was designed for 12 weeks and Experimental group I (n=15) underwent Yoga practices, Experimental group II (n=15) underwent brisk walking for 5 days per week for 12 weeks, group III acted as Control group, they were not allowed to involve any type of physical activities. Yoga and brisk walking were selected as Independent variables. Systolic and diastolic blood pressures were selected as dependent variables. The selected physiological variables were assessed by using the digital sphygmomanometer. Pre test and Post test were conducted on selected physiological variables. The collected data were analysed by using Dependent ‘t - test’ and Analysis of covariance (ANCOVA) was applied to find out the effect of Yoga and brisk walking on selected physiological variables and scheffe’s Post hoc method was used for testing the significance between paired adjusted means. The level of significance was 0.05. The results of the study indicated that the the effect of yoga and brisk walking had significantly improved the systolic and diastolic blood pressure of Non Insulin Dependent Diabetic Patients

(1,2,3,4-Tetra­hydro­isoquinoline-2-carbo­dithio­ato-κ2 S,S′)(thio­cyanato-κN)(tri­phenyl­phosphane)nickel(II)

The NiII atom in the mononuclear title compound, [Ni(C10H10NS2)(NCS)(C18H15P)], exists within a S2PN donor set that defines a distorted square-planar geometry. A significant asymmetry in the Ni—S bond lengths support the less effective trans effect of SCN− over PPh3

ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY

Objective: The aim of this study is to evaluate the anti-inflammatory activity of Nigella sativa silver nanoparticles (NS AgNPs). Methods: Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, in vitro anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay. Results: The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract. Conclusion: From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders